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mouse monoclonal antibody against gephyrin (Synaptic Systems)

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Synaptic Systems mouse monoclonal antibody against gephyrin

Scoring of synaptic cleft of inhibitory synapses as normal (A) , open (B) or unopposed (C) . Images from 3 weeks old-dissociated hippocampal cultures labeled for <t>gephyrin,</t> a GABAergic synapse marker, to delineate the postsynaptic membrane. If the pre- and post-synaptic membranes maintain the uniform gap at the edges of the synaptic cleft, those edges will be scored as “normal” (marked as “1” & “2” in A , and “3” in B ). If the presynaptic membrane is separated from the postsynaptic membrane at the edge of the cleft, that edge will be scored as “open” (hollow arrow in B ). If the gephyrin-labeled membrane is not apposed by a presynaptic terminal, it is scored as “unopposed” (C) . Scale bar = 100 nm

Mouse Monoclonal Antibody Against Gephyrin, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+antibody+against+gephyrin/mouse+anti+gephyrin/pmc11494804-34-0-18
Average 90 stars, based on 1 article reviews

mouse monoclonal antibody against gephyrin - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions"

Article Title: Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions

Journal: Molecular Brain

doi: 10.1186/s13041-024-01151-0

Figure Legend Snippet: Scoring of synaptic cleft of inhibitory synapses as normal (A) , open (B) or unopposed (C) . Images from 3 weeks old-dissociated hippocampal cultures labeled for gephyrin, a GABAergic synapse marker, to delineate the postsynaptic membrane. If the pre- and post-synaptic membranes maintain the uniform gap at the edges of the synaptic cleft, those edges will be scored as “normal” (marked as “1” & “2” in A , and “3” in B ). If the presynaptic membrane is separated from the postsynaptic membrane at the edge of the cleft, that edge will be scored as “open” (hollow arrow in B ). If the gephyrin-labeled membrane is not apposed by a presynaptic terminal, it is scored as “unopposed” (C) . Scale bar = 100 nm

Techniques Used: Labeling, Marker, Membrane

Examples of inhibitory synapses from hippocampal organotypic slice cultures (A) and dissociated cell cultures (B) . In slice cultures, inhibitory synapses (the presynaptic terminal of which was marked as “I”) were typically located on dendritic shaft, while excitatory synapses (marked as “E”) were on spines, with a distinctive PSD. (B) In 3 weeks old-dissociated hippocampal cell cultures, an inhibitory (I) synapse was labeled by a gephyrin antibody, a GABAergic synaptic marker. The same dendrite also exhibited an excitatory synapse (E) with a distinctive PSD (the edges of which marked by two arrows). Scale bar = 100 nm

Figure Legend Snippet: Examples of inhibitory synapses from hippocampal organotypic slice cultures (A) and dissociated cell cultures (B) . In slice cultures, inhibitory synapses (the presynaptic terminal of which was marked as “I”) were typically located on dendritic shaft, while excitatory synapses (marked as “E”) were on spines, with a distinctive PSD. (B) In 3 weeks old-dissociated hippocampal cell cultures, an inhibitory (I) synapse was labeled by a gephyrin antibody, a GABAergic synaptic marker. The same dendrite also exhibited an excitatory synapse (E) with a distinctive PSD (the edges of which marked by two arrows). Scale bar = 100 nm

Techniques Used: Labeling, Marker

Adherence junctions (boxed area) were often seen adjacent to inhibitory synapses ( A , C ), and occasionally at excitatory synapses ( B , D ). All examples here were from 3 weeks old-dissociated cell cultures. The distance of the gap between the two membranes of the adherence junction was narrower than synaptic cleft in both type of synapses. Label for gephyrin (C) , a marker for GABAergic inhibitory synapse, clearly delineate the postsynaptic membrane next to the adherence junction. Label for synaptophysin (B) , a SV membrane protein , and label for Shank (D) , a PSD scaffold protein , specifically labeled the pre-and postsynaptic side of excitatory synapses. Scale bar = 100 nm. (E) Occurrence frequency (%) of adherence junctions in inhibitory vs. excitatory synaptic profiles from three experimental systems, 2 experiments each (Supplementary Material 1: Table )

Figure Legend Snippet: Adherence junctions (boxed area) were often seen adjacent to inhibitory synapses ( A , C ), and occasionally at excitatory synapses ( B , D ). All examples here were from 3 weeks old-dissociated cell cultures. The distance of the gap between the two membranes of the adherence junction was narrower than synaptic cleft in both type of synapses. Label for gephyrin (C) , a marker for GABAergic inhibitory synapse, clearly delineate the postsynaptic membrane next to the adherence junction. Label for synaptophysin (B) , a SV membrane protein , and label for Shank (D) , a PSD scaffold protein , specifically labeled the pre-and postsynaptic side of excitatory synapses. Scale bar = 100 nm. (E) Occurrence frequency (%) of adherence junctions in inhibitory vs. excitatory synaptic profiles from three experimental systems, 2 experiments each (Supplementary Material 1: Table )

Techniques Used: Marker, Membrane, Labeling

Inhibitory synapses in dissociated cultures at 4 (C) and 5 ( A , B ) days in culture. Label for gephyrin (A) marked the postsynaptic membrane, and label for piccolo (B) and bassoon (C) marked the presynaptic active zone. Adherence junction (box in C) was also present early in development. Scale bar = 100 nm

Figure Legend Snippet: Inhibitory synapses in dissociated cultures at 4 (C) and 5 ( A , B ) days in culture. Label for gephyrin (A) marked the postsynaptic membrane, and label for piccolo (B) and bassoon (C) marked the presynaptic active zone. Adherence junction (box in C) was also present early in development. Scale bar = 100 nm

Techniques Used: Membrane

Inhibitory ( A , C ) and excitatory ( B , D ) synapses from 3 weeks old-dissociated hippocampal cultures upon 2–3 min depolarization with high K + , or NMDA treatment (E) . ( A , C ) Label for gephyrin underlie the postsynaptic membrane of GABAergic synapses and clearly delineates the edges of the synaptic cleft. Very few open clefts were present in inhibitory synapses upon depolarization, whereas the postsynaptic density (PSD, specifically labeled for homer in D; ) of excitatory synapses increased in thickness ( B , D ), and ~ 20% of the edges displayed open clefts (hollow arrows in B, D). (E) Activation of NMDA receptors (60 µM NMDA for 2 min) induced an increase in thickness of the PSD (the edges of which are marked with two solid arrows) and an open cleft (hollow arrow) in excitatory synapses (the presynaptic terminal marked as “E”). A nearby inhibitory synapse (marked as “I”) on the same dendritic shaft had normal cleft edges. Scale bar = 100 nm

Figure Legend Snippet: Inhibitory ( A , C ) and excitatory ( B , D ) synapses from 3 weeks old-dissociated hippocampal cultures upon 2–3 min depolarization with high K + , or NMDA treatment (E) . ( A , C ) Label for gephyrin underlie the postsynaptic membrane of GABAergic synapses and clearly delineates the edges of the synaptic cleft. Very few open clefts were present in inhibitory synapses upon depolarization, whereas the postsynaptic density (PSD, specifically labeled for homer in D; ) of excitatory synapses increased in thickness ( B , D ), and ~ 20% of the edges displayed open clefts (hollow arrows in B, D). (E) Activation of NMDA receptors (60 µM NMDA for 2 min) induced an increase in thickness of the PSD (the edges of which are marked with two solid arrows) and an open cleft (hollow arrow) in excitatory synapses (the presynaptic terminal marked as “E”). A nearby inhibitory synapse (marked as “I”) on the same dendritic shaft had normal cleft edges. Scale bar = 100 nm

Techniques Used: Membrane, Labeling, Activation Assay

Synaptic clefts of Inhibitory synapses are disrupted under calcium-free conditions. Label for gephyrin, a GABAergic synapse marker, lined the cytoplasmic side of the inhibitory postsynaptic membrane. Images were from 3 week-old hippocampal dissociated cell cultures treated with 5 min of EGTA. (A) showed a synapse with normal clefts on both edges, while B & C showed open clefts (hollow arrows), and D & E showed unopposed patches of gephyrin-labeled membrane, sometimes with axon terminals nearby, but not close enough to be counted as a synaptic contact. Scale bar = 100 nm. (F) Bar graphs on occurrence frequency of open clefts in gephyrin-labeled inhibitory synapses under different conditions, 3 experiments (Supplementary Material 1: Table )

Figure Legend Snippet: Synaptic clefts of Inhibitory synapses are disrupted under calcium-free conditions. Label for gephyrin, a GABAergic synapse marker, lined the cytoplasmic side of the inhibitory postsynaptic membrane. Images were from 3 week-old hippocampal dissociated cell cultures treated with 5 min of EGTA. (A) showed a synapse with normal clefts on both edges, while B & C showed open clefts (hollow arrows), and D & E showed unopposed patches of gephyrin-labeled membrane, sometimes with axon terminals nearby, but not close enough to be counted as a synaptic contact. Scale bar = 100 nm. (F) Bar graphs on occurrence frequency of open clefts in gephyrin-labeled inhibitory synapses under different conditions, 3 experiments (Supplementary Material 1: Table )

Techniques Used: Marker, Membrane, Labeling

Incubation:

Article Title: Distribution of gephyrin-immunoreactivity in the trigeminal motor nucleus: an immunohistochemical study in rats.
Article Snippet: .. Briefly, the sections were incubated at room temperature sequentially with (1) a mixture of mouse antibody against gephyrin (1:200; Synaptic Systems) and rabbit antibody against FG (1:1000; AB153; Millipore) overnight; (2) biotinylated donkey anti-mouse IgG (1:200; Millipore) for 4 hr; (3) a mixture of 10% (v/v) normal mouse serum, FITC-labeled avidin D (1:1000; Vector) and Cy5-labeled donkey anti-rabbit IgG (1:200; Millipore) for 6 hr; (4) counterstained with 1:1000 diluted PI (Nacalai Tesque) for 45 min. ..

Article Title: Distribution of gephyrin-immunoreactivity in the trigeminal motor nucleus: an immunohistochemical study in rats.
Article Snippet: .. Briefly, the sections were incubated at room temperature for 24 hr in a mixture of mouse antibody against gephyrin (1:200; Synaptic Systems) and affinity-purified against VGLUT1 rabbit IgG (1.5 lg/mL; Hioki et al., 2003) or affinity-purified against VIAAT rabbit IgG (1:750; Synaptic Systems) containing 2% (v/v) normal donkey serum (TBS-D). .. After washes with TBS, the sections were incubated with a mixture of goat anti-rabbit antibody-conjugated 1.4-nm gold particles (1:100; 2003; Nanoprobes) and biotinylated donkey anti-mouse antibody (1:100; Millipore) overnight in TBSD.

Avidin-Biotin Assay:

Affinity Purification:

Article Title: Distribution of gephyrin-immunoreactivity in the trigeminal motor nucleus: an immunohistochemical study in rats.
Article Snippet: .. Briefly, the sections were incubated at room temperature for 24 hr in a mixture of mouse antibody against gephyrin (1:200; Synaptic Systems) and affinity-purified against VGLUT1 rabbit IgG (1.5 lg/mL; Hioki et al., 2003) or affinity-purified against VIAAT rabbit IgG (1:750; Synaptic Systems) containing 2% (v/v) normal donkey serum (TBS-D). .. After washes with TBS, the sections were incubated with a mixture of goat anti-rabbit antibody-conjugated 1.4-nm gold particles (1:100; 2003; Nanoprobes) and biotinylated donkey anti-mouse antibody (1:100; Millipore) overnight in TBSD.

Image Search Results

Journal: Molecular Brain

Article Title: Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions

doi: 10.1186/s13041-024-01151-0

Figure Lengend Snippet: Scoring of synaptic cleft of inhibitory synapses as normal (A) , open (B) or unopposed (C) . Images from 3 weeks old-dissociated hippocampal cultures labeled for gephyrin, a GABAergic synapse marker, to delineate the postsynaptic membrane. If the pre- and post-synaptic membranes maintain the uniform gap at the edges of the synaptic cleft, those edges will be scored as “normal” (marked as “1” & “2” in A , and “3” in B ). If the presynaptic membrane is separated from the postsynaptic membrane at the edge of the cleft, that edge will be scored as “open” (hollow arrow in B ). If the gephyrin-labeled membrane is not apposed by a presynaptic terminal, it is scored as “unopposed” (C) . Scale bar = 100 nm

Article Snippet: Mouse monoclonal antibody against gephyrin (1:100, clone mAb7a) and rabbit polyclonal antibody against Homer 1b/c (1:250) were from Synaptic Systems (Goettingen, Germany).

Techniques: Labeling, Marker, Membrane

Journal: Molecular Brain

Article Title: Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions

doi: 10.1186/s13041-024-01151-0

Figure Lengend Snippet: Examples of inhibitory synapses from hippocampal organotypic slice cultures (A) and dissociated cell cultures (B) . In slice cultures, inhibitory synapses (the presynaptic terminal of which was marked as “I”) were typically located on dendritic shaft, while excitatory synapses (marked as “E”) were on spines, with a distinctive PSD. (B) In 3 weeks old-dissociated hippocampal cell cultures, an inhibitory (I) synapse was labeled by a gephyrin antibody, a GABAergic synaptic marker. The same dendrite also exhibited an excitatory synapse (E) with a distinctive PSD (the edges of which marked by two arrows). Scale bar = 100 nm

Article Snippet: Mouse monoclonal antibody against gephyrin (1:100, clone mAb7a) and rabbit polyclonal antibody against Homer 1b/c (1:250) were from Synaptic Systems (Goettingen, Germany).

Techniques: Labeling, Marker

Journal: Molecular Brain

Article Title: Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions

doi: 10.1186/s13041-024-01151-0

Figure Lengend Snippet: Adherence junctions (boxed area) were often seen adjacent to inhibitory synapses ( A , C ), and occasionally at excitatory synapses ( B , D ). All examples here were from 3 weeks old-dissociated cell cultures. The distance of the gap between the two membranes of the adherence junction was narrower than synaptic cleft in both type of synapses. Label for gephyrin (C) , a marker for GABAergic inhibitory synapse, clearly delineate the postsynaptic membrane next to the adherence junction. Label for synaptophysin (B) , a SV membrane protein , and label for Shank (D) , a PSD scaffold protein , specifically labeled the pre-and postsynaptic side of excitatory synapses. Scale bar = 100 nm. (E) Occurrence frequency (%) of adherence junctions in inhibitory vs. excitatory synaptic profiles from three experimental systems, 2 experiments each (Supplementary Material 1: Table )

Article Snippet: Mouse monoclonal antibody against gephyrin (1:100, clone mAb7a) and rabbit polyclonal antibody against Homer 1b/c (1:250) were from Synaptic Systems (Goettingen, Germany).

Techniques: Marker, Membrane, Labeling

Journal: Molecular Brain

Article Title: Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions

doi: 10.1186/s13041-024-01151-0

Figure Lengend Snippet: Inhibitory synapses in dissociated cultures at 4 (C) and 5 ( A , B ) days in culture. Label for gephyrin (A) marked the postsynaptic membrane, and label for piccolo (B) and bassoon (C) marked the presynaptic active zone. Adherence junction (box in C) was also present early in development. Scale bar = 100 nm

Article Snippet: Mouse monoclonal antibody against gephyrin (1:100, clone mAb7a) and rabbit polyclonal antibody against Homer 1b/c (1:250) were from Synaptic Systems (Goettingen, Germany).

Techniques: Membrane

Journal: Molecular Brain

Article Title: Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions

doi: 10.1186/s13041-024-01151-0

Figure Lengend Snippet: Inhibitory ( A , C ) and excitatory ( B , D ) synapses from 3 weeks old-dissociated hippocampal cultures upon 2–3 min depolarization with high K + , or NMDA treatment (E) . ( A , C ) Label for gephyrin underlie the postsynaptic membrane of GABAergic synapses and clearly delineates the edges of the synaptic cleft. Very few open clefts were present in inhibitory synapses upon depolarization, whereas the postsynaptic density (PSD, specifically labeled for homer in D; ) of excitatory synapses increased in thickness ( B , D ), and ~ 20% of the edges displayed open clefts (hollow arrows in B, D). (E) Activation of NMDA receptors (60 µM NMDA for 2 min) induced an increase in thickness of the PSD (the edges of which are marked with two solid arrows) and an open cleft (hollow arrow) in excitatory synapses (the presynaptic terminal marked as “E”). A nearby inhibitory synapse (marked as “I”) on the same dendritic shaft had normal cleft edges. Scale bar = 100 nm

Article Snippet: Mouse monoclonal antibody against gephyrin (1:100, clone mAb7a) and rabbit polyclonal antibody against Homer 1b/c (1:250) were from Synaptic Systems (Goettingen, Germany).

Techniques: Membrane, Labeling, Activation Assay

Journal: Molecular Brain

Article Title: Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions

doi: 10.1186/s13041-024-01151-0

Figure Lengend Snippet: Synaptic clefts of Inhibitory synapses are disrupted under calcium-free conditions. Label for gephyrin, a GABAergic synapse marker, lined the cytoplasmic side of the inhibitory postsynaptic membrane. Images were from 3 week-old hippocampal dissociated cell cultures treated with 5 min of EGTA. (A) showed a synapse with normal clefts on both edges, while B & C showed open clefts (hollow arrows), and D & E showed unopposed patches of gephyrin-labeled membrane, sometimes with axon terminals nearby, but not close enough to be counted as a synaptic contact. Scale bar = 100 nm. (F) Bar graphs on occurrence frequency of open clefts in gephyrin-labeled inhibitory synapses under different conditions, 3 experiments (Supplementary Material 1: Table )

Article Snippet: Mouse monoclonal antibody against gephyrin (1:100, clone mAb7a) and rabbit polyclonal antibody against Homer 1b/c (1:250) were from Synaptic Systems (Goettingen, Germany).

Techniques: Marker, Membrane, Labeling

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mouse monoclonal antibody against gephyrin (Synaptic Systems)

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1) Product Images from "Ultrastructural characterization of hippocampal inhibitory synapses under resting and stimulated conditions"

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